Speakers

Biography

My academic life journey is one characterized by the relentless pursuit of academic excellence and a profound commitment to research and innovation. Born and raised in Pakistan, My passion for knowledge was ignited at an early age, setting on a trajectory marked by remarkable achievements.

Among many achievements, I received the Commonwealth Award stands as a testament to my exceptional talent and dedication. This esteemed honor not only acknowledges my academic achievements but also underscores my commitment to fostering global collaboration and understanding.

In addition to my academic pursuits, I also ventured beyond borders to broaden my perspective and expertise. As a research student at the University of Brighton, I also embraced the opportunity to engage with diverse perspectives and cutting-edge research methodologies. My time at the University of Brighton has not only enriched my academic journey but has also positioned me as a leading figure in my field on the international stage.

Abstract

Nanoparticles have been reported to mitigate biotic and abiotic stresses in different fruits and vegetables. In this study, tomato fruit with black rot symptoms were collected and diagnosed. To control this disease, mangane­se oxide nanoparticles (MnO NPs) were synthesized in bacterial broth culture. Based on microscopic, morphological, and genetic analyses, the pathogen causing black rot disease was identified as Aspergillus niger. MnO NPs were successfully synthesized in broth-culture of Bacillus subtilis, following the process of calcination and characterized. Fourier transform infrared (FTIR) spectrum revealed the existence of stabilizing and reducing agents (carboxylic acid, alkenes, and alkyl halides) on the surface of MnO NPs. X-ray diffraction (XRD) analysis revealed the size (39 nm) and crystal-like nature of synthesized MnO NPs. Energy-dispersive X-ray spectroscopy (EDX) described the mass percentage of manganese (26.4%) and oxygen (23.3%). Scanning electron microscopy (SEM) displayed the nearly spherical shape of MnO NPs and confirmed their nano-size. These MnO NPs exhibited significant mycelial growth inhibition of A. niger and notable control of tomato black rot disease of tomato. Though, all concentrations exhibited considerable effects, medium concentrations of MnO NPs (2.5 mg/mL) performed best in both in vitro and in vivo analyses. At this concentration, tomato fruit maintained a higher percentage of soluble solids, total sugars, reducing sugars, and fruit firmness. These results proved a very effective application of bacteria-supplemented MnO NPs for the control of black rot disease of tomatoes. To our knowledge, this is the first study of tomato black rot, caused by A. niger in Pakistan.