Speakers

Biography

Beilei Wu works at the Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), china: her research interests are the Collection, diversity, and evaluation of Trichoderma spp. and the Investigation of the fermentation process for Trichoderma chlamydospore production and industrialization, Identification and evaluation of Mycovirus from Trichoderma. spp, Interactions among Trichoderma spp., pathogen and plant, Collection resource and evaluation on endophytes from the plant.

Abstract

Trichoderma spp. are known to impact host plant growth and defense activities. With the findings of the mycoviruses from Trichoderma spp., more and more effects on the sporogenesis and biocontrol functions of hosts induced by mycoviruses were known. In our research, Trichoderma harzianum partitivirus 2 (ThPV2) from T. harzianum strain (T673) showed higher mycelial density, conidiospore, and chlamydospore production than in the virus-infected strain T673-F, as well as moderately but statistically significantly improved biocontrol activity when compared with strain T673-F in the experiments with cucumber seeds inoculated with Fusarium oxysporum f. sp. cucumerinum. Trichoderma harzianum mycovirus 1 (ThMV1) from T. harzianum strain (T525) not only affected the phenotype of the host strain but also reduces its biocontrol function on F. oxysporum f. sp. cucumerinum. Using the yeast two hybrid nuclear system to screen, 57 proteins involving the interaction between T525 and ThMV1 have been obtained, among which 40 proteins interacted with ThMV1-RdRP, 29 proteins interacted with ThMV1-CP and 12 proteins interacted with both ThMV1-RdRP and ThMV1-CP. For the hexokinase1 (HXK) and the interacted protein with ThMV1-CP, the hexokinase gene (hxk) knockout mutant (T525^-hxk) and reverse mutant (T525^Rhxk) of T525 were obtained. Connecting the results of transcriptomic analysis, Real-time RT-PCR, and the phenotype of cucumber, the interaction of ThMV1-CP/HXK in T525 was proved to negatively regulate the disease resistance pathway of T525 and positively regulate the growth promotion effect of T525 in the cucumber.