3rd Edition Of Plant Science and Molecular Biology World Conference 2026

Abstract

Fusarium wilt of banana (FWB) seriously threatens the banana industry's sustainable development. One of the most effective measures to prevent FWB is breeding for disease-resistance cultivars. Recent evidence indicates that FWB occurrence and development are not only regulated by host resistance genes, but are also significantly influenced by host associated microbiomes. However, the relationship between genotype-associated microbiomes and their host linked to FWB resistance has yet to be explored. Therefore, we used the amplification sequencing method to characterize the bacterial and fungal communities in three root compartments (root peripheral, rhizosphere, and endosphere) of six banana genotypes(‘Pahang’, ‘GCTCV119’, ‘Yunjiao No.1, Nantianhuang’, ‘Baxijiao’ and ‘Guijiao No. 1’ with its different levels of FWB resistance. We isolated bacteria and fungi linked to FWB-resistant genotype biomarkers to verify their disease-suppression effect. Sequence analysis showed that the banana host’s resistance to FWB significantly correlated with its associated bacterial and fungal communities. The resistant and susceptible genotypes recruited different types of microbiomes where Bacillus, Penicillium, and Trichoderma were enriched in the root periphery of resistant genotype, whereas Flavobacterium, Sphingobium, and Pseudomonas were enriched in the root periphery and rhizosphere of susceptible genotype. Furthermore, we compared the infection processes in the susceptible cultivar ‘Baxijiao’and the  resistant cultivar ‘Yunjiao No.1’ both inoculated with pathogen tropical race 4 (TR4). The results showed that TR4 hyphae could rapidly penetrate the cortex into the root vascular bundle for colonization, and the colonization capacity in‘Baxijiao’was significantly higher than that in ‘Yunjiao No.1’. Furthermore, we have fully sequenced and assembly the genome of  ‘Yunjiao No.1’and some resistant genes to TR4 have been identified and verified. Also we found that Bacillus velezensis YN2111’s metabolic composition exhibited biocontrol properties, including promoting banana growth and inhibiting TR4 in vitro. However, greenhouse pot experiments and laser scanning confocal microscopy revealed that YN2111 could exacerbate FWB by facilitating the entry of TR4 into the plant roots. We subsequently identified a key gene involved this process that encodes banana amine oxidase, designated as MuAo. Finally, we discussed a novel interaction among biocontrol agent, banana, and TR4, with the outcomes of our research offering a better understanding of host genotype-biocontrol agent-pathogen interactions, and providing new insights into the exploration of resistance genes against FWB.