3rd Edition Of Plant Science and Molecular Biology World Conference 2026

Abstract

The growth and development of explants is governed by the composition of culture medium. Ammonium nitrate (NH4NO3) as a major salt of stock solution-1 for the preparation of Murashige and Skoog (1962) medium. But, it has several demerits on human civilization. It is use for the preparation of bomb and other destructive activities. A new chemical was identified as a substitute of ammonium nitrate. The concentrations of the other ingredients of major and minor salt were also modified from the MS medium (1962). The formulation of new medium is totally different from the MS (1962) nutrient composition. The MS (1962) medium was used as first check treatment and MS powder (Duchefa Biocheme, The Netherland) was used as second check treatment. The experiments were carried out at the Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh. Two potato varieties viz. Diamant and Asterix were used as experimental materials. The regeneration potentiality of potato onto new medium was best as compare with the two check treatments. The traits like-regeneration percentage, node number, leaf number, shoot length, root lengths were highest in new medium. The plantlets were healthy, robust and strong as compare to plantlets regenerated from check treatments-1. The regeneration and plantlet production potentiality of the new medium was validated with some other crops viz. Gerbera, Sweet potato and Aloe vera. The new medium showed excellent performance in respect of all trails under studied. Hence, the innovation of the new medium can be utilized for regeneration and large scale plantlet production of any other crops.