3rd Edition Of Plant Science and Molecular Biology World Conference 2026

Abstract

The improvement of rice crop through genetic engineering could be performed expeditiously by having reproducible regeneration protocols for different varieties. We have developed procedures for high throughput identification of embryogenic calli and developed strategies for regeneration using a recalcitrant local varieties such as ASD16 (suitable for aerobic cultivation), CR1009 (drought resistance) and Navara (medicinal properties) were selected.  The regeneration efficiency was monitored and compared using different regeneration media namely, RI, RII, RIII, RIV, RIV’, RV, RVI and RVII. Histological evidences and scanning electron microscopy for the growing embyogenic and regenrating calli.

The scutellum derived calli of varieties CR1009(non recalicitrant) and Navaran (moderately recalcitrant) and ASD16(recalcitrant) were transferred to   regeneration media RII (MS basal supplemented with 2.5 mg/l proline,  1 mg/l NAA (naphthalene acetic acid), 3 mg/l BAP (6-Benzyladenine). The variety ASD16 showed regeneration capacity by partial desiccation treatment of 24hr. Selective improvement in regeneration in ASD16 by ~30%, Navara by 23% but none for  CR1009 varieties. Desiccation treatment for different time interval prior to regeneration showed desired effect prolonged period ie, 24hr and 48hr desiccation is efficient for ASD16. The regeneration and callus were subjected to SEM, and microscopic examination of the calli. This is first report based on systemic varietal plasticity and its detailed time kinetics. This is first step to bring the recalcitrant scutellum derived calli derived from indica varieties on the same media with efficient regeneration capacity.